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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Amazônia Oriental; Embrapa Pantanal; Embrapa Suínos e Aves. |
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Data corrente: |
09/05/2016 |
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Data da última atualização: |
10/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HADI, S. I. I. A; SANTANA, H.; BRUNALE, P. P. M.; GOMES, T. G.; OLIVEIRA, M. D. de; MATTHIENSEN, A.; OLIVEIRA, M. E. C.; SILVA, F. C. P.; BRASIL, B. dos S. A. F. |
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Afiliação: |
SÁMED, I. I. A. HADI, UFT; HUGO SANTANA, UFBA; PATRÍCIA P. M. BRUNALE, UnB; TAÍSA G. GOMES, UFT; MARCIA DIVINA DE OLIVEIRA, CPAP; ALEXANDRE MATTHIENSEN, CNPSA; MARCOS ENE CHAVES OLIVEIRA, CPATU; FLÁVIA C. P. SILVA; BRUNO DOS SANTOS ALVES FIGUEIREDO B, CNPAE. |
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Título: |
DNA barcoding green microalgae isolated from neotropical inland waters. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 2, Feb. 2016. |
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DOI: |
http://dx.doi.org/10.1371/journal.pone.0149284 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences? using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker. |
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Palavras-Chave: |
Análise molecular; Identificação morfológica; Microalgas; Sequenciamento genético. |
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Thesagro: |
Alga. |
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Thesaurus Nal: |
Algae; Microalgae. |
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Categoria do assunto: |
--
P Recursos Naturais, Ciências Ambientais e da Terra
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/176636/1/journal.pone.0149284.PDF
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152647/1/journal.pone.0149284.PDF
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Marc: |
LEADER 02375naa a2200313 a 4500
001 2046770
005 2018-05-10
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1371/journal.pone.0149284$2DOI
100 1 $aHADI, S. I. I. A
245 $aDNA barcoding green microalgae isolated from neotropical inland waters.$h[electronic resource]
260 $c2016
520 $aThis study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences? using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker.
650 $aAlgae
650 $aMicroalgae
650 $aAlga
653 $aAnálise molecular
653 $aIdentificação morfológica
653 $aMicroalgas
653 $aSequenciamento genético
700 1 $aSANTANA, H.
700 1 $aBRUNALE, P. P. M.
700 1 $aGOMES, T. G.
700 1 $aOLIVEIRA, M. D. de
700 1 $aMATTHIENSEN, A.
700 1 $aOLIVEIRA, M. E. C.
700 1 $aSILVA, F. C. P.
700 1 $aBRASIL, B. dos S. A. F.
773 $tPlos One$gv. 11, n. 2, Feb. 2016.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| 1. |  | DETTMER, T. L.; ABREU, U. G. P. de; DETTMER, C. A.; ROMA, M. L.; TALARICO, T. T. Ramos do Cooperativismo: cenário da região centro oeste do Brasil. Encontro Internacional de Gestão, Desenvolvimento e Inovação (EIGEDIN), v. 6, n. 1, p. 1-12, dez. 2022. Anais do VI EIGEDIN.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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